Putative benzodiazepine receptor: A protein solubilised from brain

Chronic cocaine modifies brain benzodiazepine receptor densities.

Purification of a putative brain somatostatin receptor.

The brain somatostatin (somatotropin release-inhibiting factor; SRIF) receptor was purified by affinity chromatographic techniques. A protein of 60 kDa could be purified from rat brain. The protein was eluted from a [D-Trp8]SRIF affinity column with either sodium acetate (pH 5.5) or free [D-Trp8]SRIF. The binding of the protein to the affinity column was prevented by free [D-Trp8]SRIF or the st...

Developmental changes in protein carboxylmethylation and in the benzodiazepine receptor proteins in rat brain.

The intraventricular administration of [3H-methyl]-methionine resulted in a steady increase in carboxyl-[3H]methylated protein formation in the brain of developing rats (2-42 days). The analysis by gel filtration on Sepharose-4B of Lubrol-Px-solubilized membrane proteins, including benzodiazepine receptor proteins, revealed co-migration of carboxyl-[3H]methylated and [3H]flunitrazepam-binding p...

The gamma-aminobutyrate/benzodiazepine receptor from pig brain. Purification and characterization of the receptor complex from cerebral cortex and cerebellum.

The gamma-aminobutyrate/benzodiazepine-receptor complex has been purified from a Triton X-100 extract of crude synaptic membranes from pig cerebral cortex and cerebellum by a combination of affinity and ion-exchange chromatography. [3H]Flunitrazepam binding activity was purified 2200-fold from cortex with an overall yield of 2%. The dissociation constants for the binding of [3H]muscimol and [3H...

The Benzodiazepine Receptor

The 1,4-benzodiazepines have become the most frequently prescribed of all psychotropic drugs since the introduction of the first member of this series, chlordiazepoxide, into clinical practice in 1960. The compounds are used as anxiolytics, sedative/hypnotics, anticonvulsants, and muscle relaxants. This unique spectrum of activity, together with their large therapeutic index and essential lack ...